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maturation media 008570  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc maturation media 008570
Human cerebral organoids contain pNSCs and dNSCs throughout their development: ( A ) representative images of a d20 CO, a pNSC neurosphere, and a dNSC neurosphere. ( B ) The numbers of neurospheres per 5000 cells plated from a single CO (d20, d40, and d90 of <t>maturation).</t> There was no significant difference in the numbers of pNSC and dNSC neurospheres grown across organoid maturation days. One-way ANOVA. n = 3 independent experiments; data represent the mean ± SEM. ( C ) Individual pNSCs and dNSCs from d20 and d40 COs were passaged in the same media conditions. The number of passaged neurospheres was not significantly different between passages. One-way ANOVA. n = 3 organoids per group; data represent the mean ± SEM.
Maturation Media 008570, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maturation+media+008570/pmc11203442-120-20-23?v=STEMCELL+Technologies+Inc
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maturation media 008570 - by Bioz Stars, 2026-07
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Human cerebral organoids contain pNSCs and dNSCs throughout their development: ( A ) representative images of a d20 CO, a pNSC neurosphere, and a dNSC neurosphere. ( B ) The numbers of neurospheres per 5000 cells plated from a single CO (d20, d40, and d90 of maturation). There was no significant difference in the numbers of pNSC and dNSC neurospheres grown across organoid maturation days. One-way ANOVA. n = 3 independent experiments; data represent the mean ± SEM. ( C ) Individual pNSCs and dNSCs from d20 and d40 COs were passaged in the same media conditions. The number of passaged neurospheres was not significantly different between passages. One-way ANOVA. n = 3 organoids per group; data represent the mean ± SEM.

Journal: International Journal of Molecular Sciences

Article Title: Primitive and Definitive Neural Precursor Cells Are Present in Human Cerebral Organoids

doi: 10.3390/ijms25126549

Figure Lengend Snippet: Human cerebral organoids contain pNSCs and dNSCs throughout their development: ( A ) representative images of a d20 CO, a pNSC neurosphere, and a dNSC neurosphere. ( B ) The numbers of neurospheres per 5000 cells plated from a single CO (d20, d40, and d90 of maturation). There was no significant difference in the numbers of pNSC and dNSC neurospheres grown across organoid maturation days. One-way ANOVA. n = 3 independent experiments; data represent the mean ± SEM. ( C ) Individual pNSCs and dNSCs from d20 and d40 COs were passaged in the same media conditions. The number of passaged neurospheres was not significantly different between passages. One-way ANOVA. n = 3 organoids per group; data represent the mean ± SEM.

Article Snippet: NWL283 was diluted in ddH2O to obtain a 1000 μM stock concentration and a final concentration of 10 μM in maturation media (008570, STEMCELL Technologies, Vancouver, BC, Canada) and replaced daily in organoid cultures for 7 days, at which time the organoids were collected.

Techniques: